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To calculate I t), the total fraction of unreplicated DNA and the time distribution of origin firing were interpolated over 50 points in the interval of [10,60] minutes using a spline interpolation algorithm.
Therefore, the precocious replication of the GC-rich chromosomal arm during S-phase is not due to a higher density of replication origins nor to a peculiar distribution of origin positions along the chromosome.
Reconstruction of a nanophanerophyte (tree or treelet < 3 m tall) as the ancestor of the N4 clade says nothing about the distribution of origin(s) of the giant-rosette form within that clade.
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In fact, assuming a uniform distribution of origins implicitly considers a population uniformly distributed.
For this reason, we employed a data-driven approach accounting for intrinsic correlations in human mobility and leading to a more realistic distribution of origin-destination pairs.
It was possible that time of harvest could have an influence on the distribution of origins for juvenile scaup.
These results strongly suggest that the global distribution of origins is largely similar in all three cancer cell lines studied.
Overall, our results suggest a strong link between the distribution of origins of DNA replication and features of the genome related to gene expression and chromatin organization.
Given that the array profiles and spacing were similar in all cell lines, we wished to determine if the distribution of origins was also similar.
To study the global distribution of origins of DNA replication in human chromosomes we have followed a strategy which utilizes a DNA microarray hybridization assay to measure the enrichment of short nascent strand DNA obtained from asynchronous proliferating cells.
In addition, the spatial distribution of origins along individual chromosomes, as measured by the distributions of the inter-origin distances between consecutive origins, show no statistical difference from one chromosome to another for each of the 36 pairwise comparisons (Wilcoxon test, P values > 0.001 (adjusted threshold to account for multiple testing, Bonferroni).
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