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For the whole BBC dataset, we have also calculated the distribution of modification factors m i (Figure 5).
In most cases the extent and statistical distribution of modification have not been measured, and it has not been quantitatively demonstrated that the bioconjugates retain full bioactivity compared with unmodified protein.
The distribution of modification masses was chosen to provide significant overlap in modification mass between adjacent sets.
(C ) The distribution of modification levels for A-to-G and non A-to-G sites, for the GFL and OL tissues.
Based on the distribution of modification quality values (QV), a threshold for modification QV was increased to 50 to limit the amount of false positive modification calls (Additional file 1: Figure S19).
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The inter-inhibition between histone modifications might impose stringent control of the distribution of modifications.
In this former study the distribution of modifications typical for repressed chromatin was not included.
Once reverse transcription products have been quantified, there are now robust methods of converting the distributions of modification position into an estimate of the "reactivity" of each nucleotide to the chemical probe [ 19, 134].
Figure 3a shows the global distribution patterns of modification events in libraries.
The composite profiles revealed the similarity of modifications by displaying different distributions of modifications in a local region (Methods).
The global but uneven distribution of SUMO modification near TSSs prompted us to study the distribution of SUMO modification on different functional elements of the genome, such as promoters, coding sequences (transcripts), upstream gene regions, downstream gene regions, and intergenic regions.
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