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Additional file 1: Size distribution by intensity.

Fig. 3 a Particle size distribution by intensity of the Au-CSCaCO3NPs.

GO had the smallest size distribution by intensity and the peak was around 1,000 nm; a slightly higher peak was seen for pG.

b Zeta potential of the Au-CSCaCO3NPs showing the surface charge Table 2 Zeta potential of the Au-CSCaCO3NPs, their size distribution by intensity (d nm), and poly dispersity index (PdI) expressed in mean ± standard deviation Au-CSCaCO3NPs Peak 1 96.54 Peak 2 2.903 Peak 3 0.000 Z-Average (d nm) 57.97 PdI 0.4 Zeta Potential (mV) ± SD − 16.40 ± 3.81.

The zeta potential of the conjugated nanoparticles was done, in order to assess their surface charge, stability, and size distribution by intensity which reveals negative charge of − 16.4 ± 3.81 mV and conjugated nanoparticle average size of 57.97 nm as revealed in Fig. 3 and Table 2.

Size distribution by intensity of the scattered light was obtained using a Zetasizer Nano ZS dynamic light scattering device from Malvern Instruments.

The particle size distribution of the AgNPs was shown under different categories like size distribution by volume and by intensity.

Figure 1(a) shows the hydrodynamic size distribution of AgNPs by intensity, and the size of AgNPs was distributed up to 10 nm with mean hydrodynamic diameter 2.36 nm. Figure 1(b) shows hydrodynamic size distribution of AgNWs with mean diameter 2.1  μm.

Particle size was evaluated by intensity distribution, and particle size distribution was represented by PDI.

The particle size was presented by intensity distribution, and the size distribution was evaluated by polydispersity index (PDI).

The distribution by WT1 staining intensity 1+, 2+, 3+ was 29.3%, 19.2%, 2.0%, respectively, and almost exactly half had no staining.