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Individuals were randomly distributed on PCR plates, with three wells per plate used as negative and migration controls.
Kozachuk used rapid-scanning micro-X-ray fluorescence imaging to analyze the plates, which are about 7.5 cm wide, and identified where mercury was distributed on the plates.
The disease status of each individual genotyped was unknown to the genotyping laboratory and samples from cases and sub-cohort individuals were distributed on the plates independently of the disease status.
All serum samples were run in duplicate and randomly distributed on the plates.
In addition to failing to decrease CFU, colonies were more evenly distributed on postwash plates after use of 40% gel.
Once identified, these cells were split and evenly distributed on new plates of 70% confluent Vero cells.
Figure 2 shows the results on two toy examples, where data are two-dimensional points distributed on X Y plates.
Briefly, two-fold dilutions of tetracycline were prepared in Cation-Adjusted Mueller-Hinton 2 broth (MH-2) (Sigma-Aldrich, Switzerland) and distributed on microtitre plates.
The discrete form solution is obtained at each discrete point equally distributed on the plate.
Cells were homogeneously distributed on the plate and exhibited the typical spindle shape morphology of normal fibroblasts.
From Fig. 10, we can see that three main rupture areas (asperities) are distributed on the plate interface along the Sagami trough.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com