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In the case when you image sparsely distributed fluorescent beads in a transparent liquid there is almost no background and the objective lens itself provides a good depth resolution.
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In pea, larger appressed thylakoids are regularly arranged within chloroplasts as uniformly distributed red fluorescent bodies, while irregular appressed thylakoid membranes within bean chloroplasts correspond to smaller and less distinguished fluorescent areas in CLSM images.
The coordinates of each intensity patch are shown in (Fig. 2A) or given in the figure caption of Fig. 3. To ensure that unlabeled CaM was within the focus during laser illumination sparsely distributed green fluorescent beads (diameter: 40 nm) were used to facilitate proper focusing onto the glass surface (Fig. 3C).
In pea, we showed by CLSM that larger appressed thylakoids are regularly arranged within chloroplasts as uniformly distributed red fluorescent bodies.
These large appressed thylakoids in pea chloroplasts correspond to large, uniformly distributed red fluorescent 0.5 μm bodies seen by CLSM (cf. Figure 1A).
When Gruppo Campari began distributing the fluorescent orange herb and root liqueur Aperol in the United States in 2004, after buying it the year before, it promoted the spritz as its signature cocktail.
Whereas labeled fibrinogen and prothrombin distributed as large fluorescent structures of the size of the whole thrombus, labeling of FXa and platelets (anti-CD41 mAb) was confined to numerous 15 45 µm sized structures.
Epidermal cells of tobacco leaves transiently expressing a Zera-ECFP (enhanced cyan fluorescent protein) construct displayed well-defined and highly fluorescent foci distributed throughout the cells that were similar in size and appearance to the natural, round, 1 to 2 micron PBs present in maize seeds [ 9].
To clarify whether markers were asymmetrically distributed, we quantified the fluorescent signals of Arm, aPKC and Dlg in mutant embryos and compared it to wild-type embryos (Fig. 3).
FLAG TSP-5-1C displayed a similar ECM patterning to FLAG TSP-5-1C the absence of cytochalasin displayedistinct fluorescent puncta disimilarECMquite uniformly in the ECM arrays.
The ability to acquire images with improved axial resolution using CLSM is especially valuable for imaging thick specimens with complex fluorescent labeling distributed over a broad depth.
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