However, at water-limiting conditions, the distribution curve is skewed towards IR64-specific allele – a global distortion of preferential expression.
An obvious concern when using WTA for sensitive RNA-Seq is that the replication of transcript abundance occurs without the loss or distortion of transcript expression.
The data generated by using these methods must be extensively evaluated to determine any technique dependent distortion of the expression profiles.
However, a number of reports have been published documenting the instability of Cy5 dye to elevated ozone levels in the environment causing distortion of gene expression (Cy5/Cy3) ratios [ 6, 7].
Therefore, it is possible to consider the distortion of emotional expressions as a non-cognitive aspect that can be measured by the PEP.
Although one of the proposed hypotheses was confirmed and another one reached a marginal level of statistical significance (Crit.Av., p = .055), still we suggest that this variable be analysed in another sample that presents a higher degree of distortion to understand what really is associated with distortion of disgust expressions.
Abnormalities of the karyotype in the parental AD-iPS cells should be avoided but as in our case do not necessarily lead to a distortion of gene expression-based AD protein association networks with this approach.
For a more global assessment of the effect of distortions in expression ratios on their statistical significance, we used a scatter plot of negated log p-values (nlPv) from the limma analysis of the OneRA and the TwoRA (DRG,SN).
It is therefore difficult to exclude the possibility that cross-hybridization within miRNA families causes a distortion of quantitative estimates of expression levels of some individual mature miRNA sequences.
Importantly, we explore the effect of distortions in expression ratios on their statistical significance.
We show that especially for inducible promoters, crosstalk effects can cause a substantial distortion in the expression of proximal genes, challenging established strategies in generating transgenic animal models and tissue culture systems.
