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These markers tended to clustered on the linkage map, and a number of them showed segregation distortion (data not shown).
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All brain sections (control and treated) appeared intact with minimal cellular distortions (data not shown).
The observed Mendelian ratio for pubescent and glabrous types in the F2 population indicates a monogenic control and recessive inheritance of the trait although a slight segregation distortion was observed (data not shown).
Comparison of the biodiversity profiles from nested and single-step amplification on the same samples did not reveal any skew or distortion of the taxonomic distribution (data not shown).
We established 500 Uniprot entries per species to eliminate distortions frequently observed with very underrepresented species (data not shown).
No significant segregation distortion was detected in the other maps used herein (data not shown).
Additional experiments (data not shown) indicated that YSR suppresses sex ratio distortion compared with YEVEN by a few percent.
Since most segregation distortions affect the allele frequencies without disturbing the genotypic frequency equilibrium (non significant F value Wright fixation index; data not shown), it is probable that gametic selection was the main factor causing skewed segregation.
Segregation distortion was tested using log-likelihood ratio tests for goodness of fit to Mendelian expectations using the module SEGsort (data not shown).
Distortion from neutral expectations was detected at 41, 13, 14 and 26 loci with EW, ln RH, LOSITAN and Arlequin, respectively (data not shown).
p.i (data not shown).
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