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Expression analysis of the mature miR172 sequences and their precursors in different tissues and developmental stages might help determine where and when each miR172 member is likely to be expressed; however, distinguishing expression of individual miR172 family members using hybridization and PCR-based approaches is difficult because the four miRNAs have few sequence differences.
Distinguishing expression of a specific homoeolocus from expression of other similar sequences is difficult, in part because the sequences of homoeoloci are very similar [ 40].
It includes data from additional subjects who developed PTSD following 9/11 but then recovered, distinguishing expression profiles associated with risk for developing PTSD, resilience, and symptom recovery.
RNA-Seq offers the potential advantage of distinguishing expression levels among different members of closely related gene families with potentially different functions, whereas cross hybridization among probes often makes this a challenge using microarrays [ 25].
Therefore, without distinguishing expression status, a large number of meaningless data from nonexpressed genes would have deteriorating effects on a statistical analysis that assumed a normal distribution of data.
For each gene, we can set up a distinguishing expression value such that the signals lower than this value correspond to the lower peak in the bimodal distribution, and the signals higher than this value correspond to the higher peak.
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In May, Frank La Rue, the United Nations' special rapporteur on the freedom of opinion and expression, said that during election campaigns in South Korea, "it is very difficult to distinguish expression that is permitted from that which is prohibited".
We contrast these results with biochemically induced differentiation and distinguish expression changes associated with durable epigenetic regulation from those likely to be due to transient changes in regulation.
Thus, cDNA arrays will generally not distinguish expression from closely related duplicated sequences.
Splicing variants of UNC5C mRNA and location of the primers which can distinguish expression status of the splicing variants.
We were able to distinguish expression changes due to individual and time-specific effects from those due to food intake.
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