The assay distinguished mutations that generate fully functional, nonfunctional, and partially functional proteins.
We successfully distinguished mutations in the HBB gene by the mismatch-specific endonuclease method without need for further assay.
There are, however, limits to our ability to distinguish mutation and recombination, as we only detect recombination events that bring in DNA with a higher level of sequence divergence than that due to mutation.
We describe a method to distinguish mutation from recombination-based changes over a full genome, and this provided the data to assess the rate of mutational change, as the 7th pandemic strain N16961 was isolated in 1971, and the 6th pandemic strain, O395, was isolated in 1965.
In cases where one of the three markers showed an allelic mismatch between offspring and one of the putative parents, an additional triplet of microsatellite markers were analyzed to distinguish mutation events from extrapair paternity.
The seventh and eighth digits distinguish mutations outside the coding region.
A color code was used to distinguish mutations that are predicted to be damaging by various numbers of the methods described above (zero in dark green, one in light green, two in orange yellow, three in brown, and four in red).
This control allowed us to distinguish mutations induced by ribavirin from standing genetic variation.
Note however, that no attempt was made to determine the effect of chemotherapy in one and the same patient, and no attempt was made to distinguish mutations in the blood fraction (lymphocytes, in particular) from germline mutations.
The data presented on the mutational patterns in figures 4, 5, 6 now provides the opportunity for studies that would identify and distinguish mutations that contribute to increased neutralization sensitivity or escape.
