These antagonists bind to a distinctly separate binding site from the agonist, exerting their action to that receptor via the other binding site.
MAbs 8H2, 6F12 and 7C7 were tested against different preparations of other fungal antigens and reacted distinctly, suggesting binding to unique epitopes.
CaM-induced target dimerization has been reported as having another distinctly different binding mode.
Each NMR experiment distinctly detected binding of 6 under both set-ups 2 and 3 but not set-up 1, placing this compound as a true positive hit.
In particular, for the reaction carried out by mixing 9 equiv of ligand-to-scaffold, the sample with poor mass transport has significantly more unfunctionalized, monofunctional, and bifunctional material, which will have distinctly different binding behavior as the effective concentration is much lower and rebinding events are less favored.
The latter points to distinctly different stable binding modes, which terminate at different locations in the G31−L100 segment.
Because the rat and bovine proteins are highly homologous, particularly among the residues forming the pocket, it is unlikely that these proteins have distinctly different ligand-binding properties.
The basis for this difference was revealed by molecular docking studies, in which both of these inhibitors were shown to have distinctly different modes of binding within the ubiquinol-binding site of cytochrome b. # These authors contributed equally to this work.
In fact, a systematic analysis of such structures reveals that residues that participate in both ligand and protein binding have distinctly different characteristics from other interface residues (Davis and Sali, 2010).
The results suggest that the actions of OsWRKY13 on the expression of more than 500 genes are partitioned by different types of transcription factors through binding to distinctly distributed cis-acting elements in the promoters of OsWRKY13-upregulated and -downregulated genes.
