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In some samples, RT-PCR analysis showed that loss of protein expression was accompanied by non-random EWS-ERG RNA processing (figure 4B, lane 4), reflected by the presence of two distinct transcripts of about 250 and 500 bp.
Two distinct transcripts of DLG5 were observed differing by only 12 bp, which would predict the addition (or deletion) of 4 amino acids, but would not alter the translation frame of the gene.
Predicting the presence of differential MREs within distinct transcripts of the same gene can be rapidly and easily assessed browsing data of our computational analyses integrated in ALE-HSA21.
Successful primer sets included those for three distinct transcripts of phenylalanine ammonia lyase (PAL1, PAL2a and PAL2b), a chitinase, a glucanase, a transposable element and a DNA repair enzyme (Table 3).
Eight distinct transcripts of hemocyanin displayed molt cycle-related differential expression in this study, five of these correspond to subunits 1 3 and 5, previously isolated from C. magister.
The degradome sequencing data further supported two newly identified Eucalyptus miRNAs, which displayed two distinct transcripts of cellulose synthase as targets, detected only in the xylem sample (Eucgr.D00476.1 and Eucgr.H00939.1 in Phytozome).
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We also obtained evidence indicating that expression of PtaHB1 affected the transcriptome by altering the accumulation of 48 distinct transcripts, many of which are predicted to be involved in growth and cell wall synthesis.
Here we report that a second member of this supergene family belonging to the sigma class, is also alternatively spliced in a similar manner producing two distinct transcripts both of which have been detected in adults by RT-PCR.
Thus, spatial proximity of distinct transcripts in the transcription factories might facilitate the production of chimaeric transcripts that in turn can be used as the signal of chromosome loci contact.
When selecting the organ(s) and/or developmental stage(s) for transcriptome characterization one encounters a tradeoff between maximizing the number of distinct transcripts and maximizing coverage of individual transcripts, the two determinants of transcriptome completeness.
Therefore, the change in expression patterns of distinct transcripts suggests the requirement of different development events from UNMs to GPGs.
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