Identification of distinct template and product exit tunnels allows proposal of a detailed model for template-directed replication with minimal disruption to the circularised RNP.
Feature adaptation schemes that preserve accuracy have to be utilized in order to obtain common representations of arbitrary biometric characteristics (several approaches to extract binary fingerprint templates have been proposed, e.g., [211, 212]) allowing biometric fusion in a form suitable for distinct template protection schemes.
This resulted in the examination of four distinct template-primer complexes involving the (6 S -HNE-1 S -HNE-1, N-dGuo adduct (Chart 1).
Both women are unmarried, but they have distinct emotional templates and back stories that give them different ideas about the role men ought to play in their lives.
The resulting cDNA was submitted for limiting dilution PCR to ensure the presence of >100 distinct cDNA templates.
Plasmid prep DNA was diluted to approximately 100 distinct DNA templates and PCR amplified using Taq-Pwo enzyme mix (Expand High FidelityPLUS PCR System, Roche Diagnostics) and the high-fidelity enzyme PfuUltra II Fusion (Stratagene, La Jolla, CA).
We sequenced HIV-1 RT genes from 18 ARV-naïve subtype C-infected individuals whose plasma samples contained HIV-1 RNA levels >4.5 log copies/ml and from which we were able to extract more than 100 distinct virus templates.
The Consurf algorithm (Ashkenazy et al., 2010), which has been developed to detect conserved positions in proteins, is distinct from template-based methods, but related to our study.
Given any two sequencing traces in S, they are related either through sharing the same immediate template (traces 5 and 6 in figure 1, for example), or indirectly, through the ancestor, α, with each having a distinct immediate template.
