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Glycosyltransferases have distinct specificities that correspond to different donors and substrates.
Wild type and mutant α-lytic protease, differing by only one amino acid, have distinct specificities.
Interestingly, many PDZ domains with these three distinct specificities contained identical amino acids at positions that directly contact position 0 but differed in the loop that does not contact position 0. Computational modeling of the selected PDZ domains shows how slight conformational changes in the loop region propagate to the binding site and result in different binding specificities.
So far, more than 3800 Type II REases with 299 distinct specificities have been characterized biochemically or genetically [3].
Recent progress provided an expanded collection of recombination sites with distinct specificities, leading to Multisite Gateway technology in which more than one entry clone can be recombined into a destination vector [13].
In humans, natural bispecific antibodies are believed to be relevant to the IgG4 subclass, and half-molecule exchanges between 2 IgG4 molecules with distinct specificities are used to explain such natural bispecificity [5].
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Recent large-scale analyses of mouse [6] and human [7] data sets showed that PDZ-ligand interactions are highly specific, with distinct specificity classes evident among the binding motifs [7].
These 2 populations bear distinct specificity, stability, and genetic profiles and are differentially used in immune responses.
Moreover, a toxin is frequently associated with a number of highly homologous molecules displaying distinct specificity toward the same target.
A good example are the 17 BPP molecules found in Bj venom, each exhibiting distinct specificity toward the active sites of ACE.
MDA5, a viral double-stranded RNA (dsRNA) receptor, shares sequence similarity and signaling pathways with RIG-I yet plays essential functions in antiviral immunity through distinct specificity for viral RNA.
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