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The English school-master : teaching all his scholars, of what age soever, the most easy, short, and perfect order of distinct reading, and true writing our English-tongue... / by Edward Coote... Now the 44 time imprinted, with certain copies to write by, at the end of this book, added.
One has to be careful about interpreting the possible biases, because adaptor sequences or any fragments that appear in a distinct reading frame in a read may lead to this observation.
Another schoolmaster, Edmund Coote, of Bury St. Edmund's, in 1596 brought out The English Schoolmaster, Teaching All His Scholars of What Age Soever the Most Easy Short & Perfect Order of Distinct Reading & True Writing Our English Tongue, with a table that consisted of about 1,400 words, sorted out by different typefaces on the basis of etymology.
The English School-master : Teaching All His Scholars, of What Age Soever, the Most Easy, Short, and Perfect Order of Distinct Reading, and True Writing Our English-tongue... / By Edward Coote... Now the 44 Time Imprinted, With Certain Copies to Write By, At the End of This Book, Added.
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Briefly, a mismatched based was identified as a mutation after meeting the following criteria: (i) it was present in more than five distinct reads; (ii) the number of distinct reads containing the mismatched base was at least 10% of the total distinct reads; and (iii) it was present in <0.5% of the reads in the matched normal sample.
"Distinct reads" were defined as fragments that had different sequences at either the 5′ or 3′ end, thereby indicating that they originated from different template molecules.
The average number of reads per targeted base was 140.0 ± 23.1 for tumor samples and 157.6 ± 18.9 for matched normal DNA derived from peripheral blood, with over 90% of targeted bases having at least 10 distinct reads (Supplementary Table 2).
The OS and application files are kept completely separate, as a set of distinct read-only images.
One might speculate that the connection goes both ways - that certain characteristics are needed to achieve wealth, but that wealth itself has some distinct (read: negative) effects on a person once he or she has "made it". But it's very difficult to get at why these phenomena exist.
Distinct reads covering the same junction add significantly to a its potential to be real, but two identical reads may be from the same source, such as PCR amplification artifacts.
The distinct reads that perfectly matched soybean transcript sequences remained.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com