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By combining epitope determinates from both domains we were able to construct mutant VLPs exhibiting significantly reduced or ablated reactivity to the majority of MAbs in the panel representing five distinct reactivity classes: group, subgroup, complex and subcomplex cross-reactive, and DENV-2 specific antibodies (Table 2).
Due to the limited number of conjugation sites and the distinct reactivity of the thiol group, cysteine-based conjugation is superior to lysine-based conjugation in terms of controlled DAR and heterogeneity.
In general, the same areas of the linear sequence were targeted by IgG from the rats and rabbits, with the most distinct reactivity in both species of animals observed against peptide 2 to 5 (Figure 5A).
For I151A, the modified cluster environment is consistent with the distinct reactivity.
Both sera 19-1 and 25-1 also contained a distinct reactivity towards the SRP54N domain.
Significant progress has been made in this area, particularly in the dimetalation of internal alkynes to provide two nucleophilic sites of distinct reactivity.
Similar(48)
Specifically, a cobalt cyclopentadienyl complex of an arene dihydrodiol would be isolobal with the corresponding tricarbonyliron complex [47], yet display distinct reactivities in certain cases.
Different oxygen states exhibit distinct reactivities and provide a stimulus for the design of catalysts with specific activities in oxidation catalysis.
Rather recently, a handful of O2 dependent MNHEs with distinct reactivities and metal binding motifs have been reported, raising the questions of why most MNHEs have the facial triad and what function is associated with the unconventional metal centers.
While Ju and co-workers stress that the NMR enzyme models in the vicinity of the metal cofactors should be used with some caution, they point out the interesting possibility that the distinct active site geometries of the structures very well rationalize the distinct reactivities of Ni II)- versus Fe(II -ARD.
The implications were emphasized by confirmation that a percentage of patients with autoantibody-negative 'Type 2' diabetes have distinct T cell reactivity to islet antigens and that T cell reactivity was more closely associated with reduced C peptide than DAA 15.
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