Heterozygote breeders were used to generate distinct litters.
Nuclear counterstaining was performed with 100 ng/mL 4′,6-diamidino-2-phenylindole (DAPI) solution in PBS for 15 min. The immunohistochemical profiles of interneurons were analyzed in the somatosensory and motor neocortices of 4 pairs of mutant (Nkx6-2CreER/LacZ) and control (Nkx6-2CreER/+) littermates from distinct litters.
In contrast, the statistical analysis in panels B and C, in the same figure, were calculated based on the normalized fluorescence changes from three independent experiments (three distinct litters of mice), six coverslips (two from each mouse), and 50 boutons per coverslip, which is ∼ 300 boutons in total.
Our findings also suggest the action of distinct mechanism in controlling litter mass variations at regional and sites scales.
2010) or measured ecosystem processes directly influenced by the focal species, for example, decomposition rate of litter produced by distinct genotypes that vary in tissue biochemistry (Driebe and Whitham 2000; Madritch and Hunter 2003; Schweitzer et al. 2004).
We used five biologically distinct samples derived from individual litters, and counted a minimum of three TEB structures per slide.
We used six biologically distinct samples derived from individual litters and counted a minimum of three ductal structures per slide.
Our data, however, provides substantial evidence for a gradient in trophic level among our litter-based invertebrates rather than two distinct trophic groupings, a finding that coincides with that of Scheu & Faica [ 26].
Distinct environmental conditions at this site (no cultivation, leaf litter layer on the soil surface) enabled a more diverse oribatid community to establish itself, which was comprised of more specialized (less tolerant) species.
Leaves in the litter from the burned area were also structurally distinct – they were relatively more intact and therefore occupied a greater volume than the partially consumed leaves in the litter from the unburned area (pers.
