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To facilitate the disruption of individual lncRNAs and for eventual pooled functional screens of many lncRNAs, we devised a protocol for the simultaneous cloning of two distinct guide RNA sequences into a single lentiviral vector, which we call DECKO (Dual Excision CRISPR Knock Out) (Fig. 1b).
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While there is certainly no single road to success, there do seem to be some distinct, guiding blueprints to getting there.
If gap patterns are mostly dictated by the guide-tree, then the use of a distinct guide-tree should have a large impact on the ability of gap patterns to reconstruct the correct tree.
The initial sorting into different endosomes occurs already at the plasma membrane and is probably dependent on the endocytic receptors and adapter proteins utilized 10 and this distinct guiding of endocytosed antigen is very important for antigen presentation 9.
Distinct interview guides were prepared for the local planners, the community groups, and the consulting firm.
Segmental polyurethanes exhibit biphasic morphology and can control cell fate by providing distinct matrix guided signals to increase the chondrogenic potential of mesenchymal stem cells (MSCs).
DiCE promotes the coordination of complementary information from distinct fields to guide decisions about which findings are most worthy of follow-up efforts.
Other options include using distinct promoters and guide RNAs to avoid repetitiveness and increase stability or employing newly characterized, engineered, or evolved Cas9 variants with improved properties (Esvelt et al., 2011 ; Mali et al., 2013b ).
The internet offers a wealth of resource guides distinct to your region's soil makeup, climate and growing season, making planting and troubleshooting a snap.
The team agreed that the principles should be distinct and action guiding, and less abstract than other well-known bioethics principles such as respect for persons, beneficence, nonmaleficence and justice [26].
Either these two methods should be merged, or if it were judged that the two concepts are very distinct, then "Ask guided questions about the nature and presence of God" should be split out to two different interventions.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com