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To obtain a measure of general activity, every transition between distinct cage locations (the two perches, front, back and side walls, floor and tray) was recorded (PositionChanges).
We divided the top view image of the cage into areas that corresponded to distinct cage locations sufficiently detailed to record the bird's movements, e.g. "left perch", "tray", "water bath", "corner at left front top" (Figure 1b).
The remaining classes resulted in reconstructions that did not show distinct cage details, presumably due to the presence of misaligned, more significantly distorted or damaged particles.
The sequence of position changes (coded as distinct cage locations) was transferred from the EthoVision into the Theme software package (Noldus Information Technology) to measure recurring sequences of events (T patterns) as a measure of route tracing (for a detailed discussion of Theme and this approach see Brilot et al. 2009; Feenders & Bateson 2012).
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Varroa-parasitized and non-parasitized bees (control) were placed in distinct cages in the dark at 32°C and 70% RH and fed ad libitum with candy (30% honey, 70% powdered sugar) and water.
For the next 24-h period, mice were socially isolated within a second, distinct home cage environment (isolate-housing condition; Fig. 1c).
In contrast, mESCs expressing G9a harboring a single point mutation of the Asp905 residue located in a distinct non-cage surface of the ANK domain that interacts with histone H3 residues 10 to 13 [ 11] display more than a twofold reduction in de novo DNA methylation levels of Oct3/4, similar to the levels observed in G9a-null mESCs (knock-out; KO).
For sensitization associated with a new environment, or context dependent sensitization, the same dose of cocaine (15 mg/kg, i.p). was given to WT and KO mice for 5 consecutive days in an activity chamber that is distinct from the home cage.
These results demonstrate that weak activation of SF1+ neurons can produce withdrawal from a context distinct from the home cage.
Our system detected a total of 255,396 vocal signals, of which 199,288 were localized to a distinct position in the cage.
Make sure the cage has distinct sleeping, eating, and toilet areas.
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