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Lateral and vertical distances were calibrated using a NIST-traceable VLSI optical profiler step height standard certified to be 44.1 nm ± 0.9 nm [ 33].
Proton proton distances were calibrated using the well resolved methylene protons of the Cys (heterocycle ring) residue based on the relationship r = rref ηref/η 1/6, where r is the distance between atoms, η is the NOESY cross-peak volume, rref is the known distance, and ηref is the corresponding volume of the NOESY calibration cross-peak.
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The sample to detector distance was calibrated using a silver behenate reference sample.
All other intra- and intermolecular distance restraints were calibrated using the Aria2.3 program.
To achieve accurate particle flow simulations, parameters of the pharmaceutical spherical granules were calibrated using experimental bounced height, rolling distance and angle of repose.
Pressure signals were calibrated using brachial BP and measurements were taken of the distance of the carotid and femoral pulses from a fixed point (the suprasternal notch).
The α-anomer proved to bind more tightly, and the NOEs for this anomer were calibrated using the 2.39 Å as the N-acetyl-glucosamine (GlcNAc) H1 H2 distance.
Gene expression levels were calibrated using endogenous TBP expression levels.
High magnifications were calibrated using graphitized carbon.
Date estimates were calibrated using two age constraints.
Pd coverages were calibrated using both AES and TPD of CO.
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