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Discrimination was considered successful if the minimal interspecific distance involving a species was larger than its intraspecific distance [4].
Similarly, using Euclidean distance method, species recovery was considered successful if the minimum interspecific Euclidean distance derived from di- and trinucleotide frequencies was higher than the maximum intraspecific Euclidean distance involving a species.
Species discrimination was also calculated using the criteria reported by CBOL plant working group [17] i.e. discrimination was considered successful, if the minimum interspecific K2P (Kimura-2-parameter) distance involving a species was larger than its maximum intraspecific K2P distance.
Rausher et al. [ 11] studied evolution patterns of 6 core structural genes in the anthocyanin pathway and found that, over a broad taxonomic distance involving monocots and dicots, the upstream genes evolved substantially more slowly than the downstream genes.
Since axis proteins inhibit DSB formation within ∼800 bp of their binding sites (Ito et al., 2014), the Red1/Hop1-dependent local concentration of DSB factors likely activates hotspots at a distance, involving higher-order chromatin interactions.
In studies of evolutionary rates of genes in the plant anthocyanin pathway, Rausher et al. [ 11] and Lu and Rausher [ 13] demonstrated that upstream genes in the pathway evolved more slowly than downstream genes both over a broad taxonomic distance involving monocots and dicots and at the intragenic level between species within a genus.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com