These elementary steps engender, in the growing daughter chain, a primary structure identical to that of the distal template strand.
In a newly suggested Type VI dermoid sinus, there is an open sinus tract that extends about to the level of the supraspinous ligament but has a distal connecting strand with the dura mater [ 6].
The proximal His152 and Asp154 residues originate from the βII strand, and the distal His221 originates from the βVII strand.
In the standard library construction of the Illumina platform, in the case of no inversions or duplications, the read that maps to the proximal location is expected to be in the + strand, where its mate should be mapped to a distal location in the − strand.
Also, the adduct is well-aligned with the damaged strand, and the distal aromatic ring stacks dynamically with Phe171.
Analysis of the secondary structure with STRIDE (Frishman and Argos, 1995) suggested that Val-Ala-Met in the distal part forms a β-strand.
To quantify the extent of cell surface labeling, cells were incubated with 100 μL of a 20mer complementary 6-FAM modified oligonucleotide (1 μg/mL, Operon), which annealed to the most distal portion of the anchor strand.
The secondary-structure predictions indicate the presence of β-strands at similar topological locations as in PCP proteins: a β strand at the N-terminal end of the hydrophilic region distal to TM1 and another β strand at the C-terminus of this region proximal to TM2.
When a Chi sequence is present, a prominent band appears in the smear of cleavage products, which reflects the downregulation of nuclease activity on the 3′-terminated strand and the protection of that strand between Chi and the distal DNA end.
GENCODE gene annotation shows that the novel promoter on the plus strand is likely a distal promoter of the GCA gene, a regulator of adhesion.
