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For experiments conducted in the presence of CO2, stock solutions were made by dissolving reagent grade NaCl, SrCl2, FeCl3 and Fe(NO3 3 solids in distilled and deionized water.
Supersaturated calcite solutions were made by dissolving reagent sodium bicarbonate (NaHCO3, Aldrich) and dehydrate calcium chloride (CaCl2, Aldrich) into deionized water (18.2 mΩ).
All reagents were dissolved in sterile phosphate buffered saline (PBS) and 200 μl was injected intravenously (i.v ., unless stated otherwise.
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A sodium arsenite solution was prepared by dissolving reagent-grade As2O3(s) in a NaOH solution.
The SBF solution has been prepared by dissolving reagent-grade NaCl, KCl, NaHCO3, MgCl2·6H2O, CaCl2, and KH2PO4 into distilled water and buffered at pH = 7.25 with TRIS (trishydroxymethyl aminomethane) and 1 N HCl solution at 37°C [ 32].
For mouse vaginal application, the dissolved reagents were formulated with 1.5% hydroxyethyl cellulose (HEC) (Sigma-Aldrich, St Louis, MO, USA).
All solutions were prepared by dissolving ACS reagent grade chemicals (J.T. Baker® unless mentioned otherwise) in ultra pure water (≈18 MΩ-cm; Barnstead, Dubuque, IA).
We prepared hematin solutions by dissolving commercial reagent at 2 mM in a 0.1 M NaOH (aq), following published procedures.
Liposome colloidal suspensions (empty liposome and GemPo suspensions) were obtained by dissolving each reagent in dichloromethane, whereas Gemcitabine was dissolved in methanol using rapid stirring.
The primary functions of solvents in chemical syntheses, such as dissolving the reagents for facilitating mass transfer and modulating chemical reactions in terms of reaction activity and selectivity are all a result of solvation.
The stock solutions were made by dissolving the reagents in the bath solution (GABA) or DMSO (bicuculline and selamectin).
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