Zeta potentials of gelatin, silicate nanoplatelet, and mixtures of silicate and gelatin were determined in ultrapure water (Milli-Q) and phosphate buffered saline (PBS), pH 7.4 (Invitrogen) using a 633 nm laser in a Malvern ZEN3600 (Malvern Instruments, U.K .. Silicate nanoplatelets were dissolved with vigorous agitation (vortexing) while gelatin was dissolved with stirring at 40 °C.
To quantitatively assay nodule mineralization, the ARS stain was dissolved with cetyl-pyridinium chloride (CPC Fisher Scientificic, Ottawa, ON, Canada) for 1 h under gentle agitation.
Consistent biliverdin production was achieved with 40% dissolved O2, agitation between 280 and 500 rpm, and continuous feeding of lactose (2% wt vol-1) and glycerol (10% wt vol-1) in fed-batch mode initiated 4h after culture inoculation (exponential growth phase).
The bioprocess is performed in 100 L Bioengineering bioreactor with 42 L cultivation medium, equipped with pH, temperature, dissolved oxygen, and agitation controllers.
Gulati et al. [ 29] reduced the time period for lipase production by Aspergillus terreus from 96 h in shake flask to 54 h in a bioreactor with control of dissolved oxygen and agitation.
Walser's sentences might declare the need for obedience, order, subservience, but those declarations are dissolved in the agitations of his syntax.
Some plays dissolve with time.
The media was then removed and replaced with 150 μl of 100% DMSO to dissolve the formazan crystals with agitation for 5 10 min on a shaker.
Next, the powder was completely dissolved by heating with frequent agitation for 1 min and then sterilized at 121°C for 10 min.
Initially, the weighted amounts of Fe(NO3 3·9H2O and Co(NO3 2·6H2O were dissolved in ethanol under agitation.
The oxidized and non-oxidized fibrils were then dissolved in 80% v/v formic acid with agitation.
