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No activity was observed for dissolved solution (control).
The rheological behavior of silk fibroin solution was significantly influenced by HAp contents in dissolved solution.
At the same time, silk fibroin nanofibers were observed in dissolved solution with 103.6 ± 20.4 nm in diameter.
To get round these issues, the company has developed what Mr Bilton describes as a "liquid catalyst" composed of "a dissolved solution of metal salts".
Then, the dissolved solution was stirred for 5 h at 80°C and dried in the oven to form the precursor at 140°C.
Then, the dissolved solution was stirred for 4 h at 80°C and dried at 80°C in the oven to form the precursor.
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After incubation at 37°C for 10 min, the absorbance of the dissolved solutions was detected at 490 nm on a microplate ELISA reader (Thermo Labsystems, Japan).
Finally, the dissolved solutions were adjusted to a volume of 25 mL with 3% HNO3 in water and used for ICP-MS analysis.
The hydrogels were formed by mixing separately dissolved solutions of the polymer and NaIO4 (both at double the final concentration) in 10 mM sodium phosphate buffer adjusted to a pH of 5.7, 6.7, 7.4, or 8.0.
The lower density solutions (ρ1~ ρ7) were prepared from ρ0 solution by diluting the dissolved Ficoll solution with PBS.
Under agitation, 3.76 g of phenol and 0.5 g of ammonium chloride were dissolved in solution.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com