The ratios of light excited at 340/380 nm and emitted at 520 nm were converted to pH using a calibration curve established using 10 μM of the H+/Na+ ionophore monensin, and 20 μM of the H+/K+ ionophore nigericin dissolved in 20 mM Mes, 110 mM KCl, and 20 mM NaCl adjusted to pH 3.0 7.0 with HCl/NaOH.
After serum-free starvation for 4 h, cells were incubated with vehicle or ME (dissolved in DMSO) at various concentrations for 30 min and then stimulated with vehicle or 10 nM insulin (dissolved in sterile water with pH 4.0) for 5 min.
ME was dissolved in chloroform.
Dishes (60 mm) were coated with 1% SeaPlaque agarose (BioWhittaker Molecular Applications, Rockland, ME, USA) dissolved in DMEM/10% FBS.
BSB-Me was dissolved in THF (2 mM) at 50°C, and 100 μl of the solution was injected into vigorously stirred (1,500 rpm) poor solvent water (10 ml at 24°C) using a microsyringe.
As a control for successful crosslinking, 5 µg of lysate samples were dissolved in SDS-sample without β-ME.
So, for example, a section about Cavendish's struggle to conceive a child consists largely of a list of the cures that were prescribed to her and her husband: This time they tried, for him, crystals taken from wood ash and dissolved in wine each morning; for me, a tincture of herbs put into my womb at night with a long syringe.
As the candy dissolved in my mouth, Marilla asked me questions, many of which weren't related to the study.
I was six years old when Richard Nixon -- my president, the president chosen for me -- dissolved in a sniveling puddle on national television, disgraced.
Tin Machine dissolved in 1992.
It officially dissolved in November 2009.
