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When all the sucrose has been dissolved, carefully pour the sucrose and water solution into the plastic bucket or glass carboy.
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The dry extracts were carefully dissolved in 0.2 ml assay buffer [ 16].
The pellet was carefully dissolved in 500 μL of 50 mM ammonium bicarbonate and quantified using a Quant-iT™ Protein Assay Kit (Invitrogen, USA).
Then dried methanol extract (4 mg) of M. fraxinea Sm. was first dissolved in methanol and carefully applied by pipette at the top of prepared column.
Uniform size filter paper disks (3 disks per compound) were impregnated by equal volume (10 μL) from the specific concentration of dissolved tested compounds and carefully placed on inoculated agar surface.
The test was performed on medium potato dextrose agar (PDA) which contain infusion of 200 g potatoes, 6 g dextrose and 15 g agar [29] Uniform size filter paper disks (3 disks per compound) were impregnated by equal volume (10 µL) from the specific concentration of dissolved tested compounds and carefully placed on inoculated agar surface.
Add salt and brown sugar, and heat just until milk is hot enough to dissolve sugar, watching carefully.
For a quick fix: carefully dissolve gelatin in concentrated liquid potpourri to make your air fresheners.
The pellets were carefully drained and dissolved in 6 M guanidine hydrochloride with 20 mM sodium phosphate buffer, pH 6.5.
Gel bands of fragments at about a 100 bases were carefully cut out and dissolved in 0.4 M NaCl at 70°C.
[Ag(CO 2(CF 2) 2CF 3)TMP] (3): Silver(I) heptafluorobutanoate (166 mg, 0.517 mmol) was dissolved in acetonitrile (2 mL) and carefully layered on a solution of TMP (75 mg, 0.550 mmol) in acetonitrile (2 mL).
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