Ideally the chemical agent is dissolved directly in physiological saline.
Hydroxyethylcellulose (HEC) was then dissolved directly (2% w/v) into the various diluted stocks using a roller mixer (Denley Spiramix 5) until homogenous gels were formed.
T2151, Sigma-Aldrich Co. LLC., USA) solution were dissolved directly in two ml of 4% sulfuric acid in methanol, vortexed for 30 s, and were then methyl-esterified at 80 °C for 1 h.
Gypsum was mixed with upper 1 cm of soil column prior to leaching at application rates of 0, 5, 10, 15, 20, 25, 35, and 40 Mg ha−1 (Mg ha−1 is mega grams or metric ton per hectare), whereas calcium chloride was dissolved directly in irrigation water and applied at equivalent application rates of 0, 4.25, 8.5, 12.75, 17.0, 29.75, 29.75, and 34 Mg ha−1.
Phosphogypsum was mixed with top soil prior to leaching at application rates of 5, 10, 15, 20, 25, 35, 40 Mg ha−1, whereas calcium chloride was dissolved directly in water at equivalent rates of 4.25, 8.5, 12.75, 17.0, 29.75, 29.75, and 34 Mg ha−1, respectively.
The hemolymph usually lacks any respiratory pigment, although members of the families Arcidae and Limidae are known to possess haemoglobin dissolved directly into the serum.
Peptide No. 12 was also available as preformed 1% hydrogel (PuraMatrix, BD Biosciences, San Jose, CA) and had been dissolved directly in water.
MLE were added to a final concentration of 10 mM dissolved directly in Incomplete Freund's adjuvant.
Embryos were deyolked as described previously [31], and dissolved directly by 2×SDS-PAGE loading buffer (2 µl per embryo).
