Exact(1)
Although the optimized path for the ADP release with Mg2+ ions resembles the path obtained without Mg2+ in previous SMD simulations, with the adenine ring leaving the binding site first (51), our free energy analysis suggests a different dissociation sequence.
Similar(7)
Selected proteomic markers (based on significance score determined by SAM) were semi-purified on ProteinChip arrays and identified directly on spots by collision-induced dissociation sequencing.
Collision-induced dissociation provided sequences information by producing characteristic glycosidic cleavage ions.
Samples were subjected to MALDI-MS profiling with major peaks selected for collision-induced dissociation and sequencing by tandem mass spectrometry.
Peaks from the H2B intact mass spectrum were then fragmented using electron transfer dissociation (ETD) for sequence analysis.
Patterns of dissociations were studied in further analyses (first value = direction of dissociation between automatized sequences of numbers vs. letters; second value = direction of dissociation between automatized sequences of numbers vs. months).
DNA methylation was analyzed by bisulfite sequencing, dissociation curves, and HumanMethylation450K arrays.
The specificity of primer pairs (Additional file 1) used in this study was determined via dissociation curves and sequencing of amplified PCR fragments (data not shown).
Related(1)
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