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As an alternative to the gentle dissociation procedure, colonies of H1 hESC can be fully dissociated into single cells by TrypLE.
For a high proportion of genes tested, the dissociation procedure itself increased expression such that the detection of BDNF-related induction could no longer be measured.
Additionally, because HCN channel isoforms are differentially regulated by various intracellular messengers [47], they may exhibit different sensitivity to damage or disruption of regulatory processes during the dissociation procedure.
A dissociation procedure was performed to generate a melting curve for confirmation of amplification specificity.
Single SAN cells were isolated from 42 rabbit hearts by an enzymatic dissociation procedure as previously described [ 42].
Indeed, the dissociation procedure reported here is not optimized for astrocytes as the mechanical disruption decreases astrocyte viability, reducing their recovery.
Similar(48)
For one case (chondroblastoma 13; CB 13), primary cells were isolated from the tumour by using mechanical and enzymatic dissociation procedures.
To achieve an array format compatible with majority of commonly used adherent cell types, different array surface materials were tested in combinations with different ECM protein coatings, cell dissociation procedures and adhesion incubation times for different cell types.
Methods like Jacoby's Process-Dissociation Procedure and Sherman's QUAD Model.
Which is where Jacoby's process-dissociation procedure (PDP) comes in.
In another study, David et al. (2000) applied a variant of Jacoby's Process-Dissociation Procedure to posthypnotic amnesia.
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