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Neuronal cultures were prepared by incubating dissociated tissue with 0.25% trypsin for 30 min, neutralized with 10% fetal bovine serum, and further dissociating by triturating.
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Cultures were passaged by dissociating spheres by mechanical trituration.
Day 14 EBs were dissociated by treatment with Accumax™ Solution (Sigma) at 37 °C followed by mechanical dissociation (as described previously27).
Digested RICs were dissociated by 23 G syringe pumping several times.
Atomic oxygen is simultaneously generated when molecular oxygen is dissociated by 184.9nm and ozone by 253.7nm.
However, because most miRNA duplexes contain bulges to prevent cleavage, the miRNA* strand dissociates by unwinding14,15,46.
The four Br atoms have been successively dissociated by voltage pulses from the tip.
The two Br atoms have been successively dissociated by voltage pulses from the tip.
Explicit and implicit adjustments were dissociated by a series of posttests.
Two days after the final treatment, tumours were dissociated by collagenase digestion and subjected to TUNEL apoptosis assay.
First, protodimers are formed, which can be dissociated by changing the pH back to 8.
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