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Remarkably, when tranilast was added to mammospheres that were already formed, at concentrations ≥100 µM, they were completely dissociated within 48 hours.
Here, we show that when tranilast was added to mammospheres that were already formed, generated from mitoxantrone-selected MDA-MB-231 (human) or 4T1 (mouse) cells, the spheres dissociated within 48 h (Fig. 4A).
If spheres are not dissociated within a week they become cystic and resistant to disaggregation by trypsinization.
Tumor fragments were either dissociated within 2 hours after surgery (28 out of 49, 58%), or incubated in organotypic conditions for a maximal time of 2 weeks prior dissociation.
Similar(56)
For example, poly(caprolactone)- b-poly ethylene oxide) and poly(d, l-lactide)- b-poly ethylenethylene glycoxideicelles were found to dissociand within 1 h under biological conditions.
All protein that did not dissociate within this period from the membrane was stripped off by a 20 s pulse injection of 50 mM NaOH.
Typically, such decreased PL quenching is obtained when the donor/acceptor interfacial area is decreased, because less photogenerated excitons can reach a D/A interface to dissociate (within their small diffusion radius) and instead recombine radiatively.
Since anxiety behavior and spatial behavior may dissociate within the hippocampus [ 2], an intriguing question that awaits clarification is why core symptoms of clinical anxiety manifest phenomenologically with strong spatial referents, as exemplified in agoraphobia.
Undifferentiated skin stem cells within non-adherent spheres (Figure 1A, a) were dissociated, and within 24 hr of plating in differentiation medium, the stem cells were sparsely attached to the 60-mm culture dishes, adopting a "fibroblast-like" morphology (Figure 1A, b).
Therefore atrial tissue is dissociated with collagenase and the dissociated cells of interest (cardiac fibroblasts) adhere rapidly to cell culture plates, for example, within 3 h of plating.
Within 1 week, when cultures were 60 70% confluent, the cells were dissociated with 0.25% Trypsin-EDTA (Invitrogen) and transferred to a new dish for further expansion.
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