Briefly, hippocampal neurons were dissociated using dissociation solution and cultured using the glial-conditioned medium, in a glass-bottomed culture dish (Matek Corp ,USA).
Tumorspheres were dissociated using non-enzymatic dissociation solution (Sigma) and strained over a 40 µM filter.
Cells were then dissociated using the Papain Dissociation System (Worthington Biochemical Corporation, Lakewood, NJ) according to the manufacturer's protocol.
The tissue was then dissociated using a Papain dissociation system (Worthington Biochemical Corp ,Lakewood, NJ, USA), using incubation times from 50 70 min.
The cerebella were dissociated using a papain-based dissociation kit (Worthington Biochemical Corporation).
For Embryoid Body (EB) formation, iPSC were dissociated using enzyme free cell dissociation buffer, resuspended in LIF free medium and cultured at low attachment condition.
Exosomes were dissociated using Total Exosome RNA and Proteins Isolation kit (Invitrogen) according to the protocol.
After cell expansion, the hydrogels were dissociated using the thiol disulfide exchange reaction in the presence of N-acetyl-cysteine or glutathione, which dissolved the hydrogel network.
Briefly, ESCs were dissociated using 0.1% trypsin-EDTA.
Prior to plating, the ganglia were dissociated using a fire-polished Pasteur pipette.
After incubation for 30 minutes at 37°C, hepatocytes were dissociated using a 5-ml syringe.
