HCC tumours from mice were excised, minced and enzymatically dissociated with type I collagenase (200 U/ml) and DNase (270 U/ml).
Five hours after EdU injection midguts were dissected and cells dissociated by collagenase (type I; Sigma) treatment.
The fat pads were then further enzymatically dissociated with collagenase Type I (Gibco, Life Technologies, Paisley, UK) and finally filtered through a 100- μm BD Falcon Cell Strainers (BD Bioscience, Oxford, UK) to remove debris.
Compound- or vehicle-treated tumours were cut into 2 mm pieces and enzymatically dissociated with collagenase type XI (1 mg/ml in H2O, Sigma) for 15 45 min at 37 °C.
Minced tissues were then dissociated with collagenase type A (1 mg ml 1; Roche Applied Science, Penzberg, Germany), dispase (1 mg ml 1; Invitrogen) and deoxyribonuclease I (100 units ml 1; Sigma-Aldrich) at 37 °C with gentle agitation for 3 4 h in HBSS with 1% fetal calf serum.
(F ) Typical neurospheres after 8 days culture from E12.5 or E18.5 dorsal telencephalon cells dissociated from wild-type or Imp1 β-geo/β-geo mice.
When SoFa retinas are dissociated, however, neuronal types are immediately identifiable independently of their morphology, by virtue of their fluorescent spectra (Fig. 6A-R).
On solid media, the bacteria of this vaccine strain are dissociated into two types of colonies, the blue and grey variants (80%and20%0%, respectively).
Isolated diaphragm muscles were dissociated from wild-type, mdx and mdx/IL6 mice both mechanically, by mincing them into a coarse slurry with scissors, and enzymatically, using 0.2% collagenase type II (Sigma) in PBS for 90 min at 37°C (65).
Although adsorbed FMN cannot participate in mediated electron transfer (MET -type bioelectrocatalysis, dissociated FMET -typeution works as a mediator for MET-type bioelectrocatalysis of the HCOO− oxidissociatedlanar electrodes.
Elemental analysis is useful for the quantitative analysis of the components of calcium phosphate crystals but the crystals need to be dissociated prior to this type of assay.
