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To determine the potential proliferation and differentiation capacities of aged NSC/NPCs in the SEZ/SVZ, we micro-dissected walls (EZ together with SEZ/SVZ) of lateral ventricles covering striatum and dissociated them into single cells (Fig. 1A).
To isolate the cells that express IRBP-GFP from the ES cultures, we dissociated them into a single-cell suspension using trypsin.
We developed cells on phosphate agar plates, harvested them after 10 to 12 hours and mechanically dissociated them into single cells.
NH4Cl did not alter the distribution of ribosomal protein S6 (RPS6), while addition of EDTA to pelleted polysomes dissociated them, as expected (Supplementary Figures S1B and C).
In order to determine the possible effect of GSI on the NSCs self-renewal ability, we harvested the spheres and dissociated them into a single cell suspension by soft pipeting.
To assess whether the PHBECs and hAMSCs preserved their molecular identities when grown as monocultures on coated meshes after prolonged ex vivo culture, we dissociated them after 30 days ex vivo culture, used FACS to sort viable cells, and analyzed the global transcriptome of these cells.
Similar(51)
Because mNSCs proliferate in neurosphere forms, they were passaged every three days using the NeuroCult Chemical dissociation kit (StemCell Technologies) or mechanical trituration to dissociate them into single cells.
He dissociates them from the operas by emphasising the comparative severity of their instrumental world, yet at the same time emphasises the close relationship between text, sound and atmosphere.
By the end, both Jason and Margery learn to harmonize their ids with their egos, acknowledging their desires instead of dissociating them in the form of lust or sock puppetry.
Although much digital activism falls into the category of electronic civil disobedience, some activists ask that such online political gestures always represent a communal interest and not an individual agenda and that their motifs and agents be public knowledge so as to dissociate them from acts of cyberterrorism or criminal hacking.
However, some droplets were connected in clusters and a watershed function was used to dissociate them.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com