The experiments in closed system also demonstrate that the dissociating system approaches a meta-stable state with a pressure much lower than equilibrium dissociation pressure.
For dissociated cultures embryonic cortices were also cut into small pieces and dissociated with the Papain Dissociation System (Worthington Biochemicals) according to the manufacturer's instructions.
The tissue was then dissociated using a Papain dissociation system (Worthington Biochemical Corp ,Lakewood, NJ, USA), using incubation times from 50 70 min.
Samples from human tumors were dissociated with the Papain Dissociation System (Worthington Biochemical Corporation, Lakewood, NJ, USA).
Cells were then dissociated using the Papain Dissociation System (Worthington Biochemical Corporation, Lakewood, NJ) according to the manufacturer's protocol.
Cultured ventral midbrains were dissociated using a Papain Dissociation System (Worthington) and cells were sorted on a Cytopeia Influx cell sorter.
Cortical neurons were obtained from embryonic day 16 (E16) mouse embryos and dissociated with the papain dissociation system (Worthington Biochemical Corp., NJ, USA) following the manufacturer's instructions.
Because you've got two almost completely dissociated operating systems, you wind up with two Web browsers, two Control Panels, two Search mechanisms, two ways of right-clicking.
For serial transplantation, brain tumors were dissociated enzymatically using the papain dissociation system (Worthington).
A Papain Dissociation System (Worthington Biochemical Corporation) was used to dissociate airway epithelial cells from the trachea as described [ 24, 25].
