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To passage hESCs, confluent culture was washed with phosphate buffered saline (PBS), dissociated for 5 min with TrypLE and resuspended into single cells with culture medium.
"I was dissociated for many years".
Keep in mind that I saw Frozen only under duress and dissociated for most of it, and I don't like kids' movies as a whole (except Dumbo), because of the scene when the mother sticks her trunk out of the jail and rocks the baby, which is a real tearjerker.
Cells were dissociated for 5 hours by gentle shaking.
Colonies were dissociated for passaging using trypsin with 3 mm glass beads.
After 8-day culture, neurosphere were formed and the cells were enzymatically dissociated for following experiments.
Similar(24)
The molecular avidity of rHIgM12 is implicit in the calculation of the dissociation rate constant because all rHIgM12-ganglioside interactions must dissociate for a molecule to leave the SPR sensor surface.
MiR-608 or -132 oligos were diluted in buffer (serial two-fold dilutions, 0.3125, 0.625, 1.25, 2.5, 5 and 10 μ m) and injected over the flow cells for 2 min at 10 μl/min, with 5-min association and 5-min dissociation, except for the highest concentration that was allowed to dissociate for 1 h.
PriL-CTD protein in buffer F containing 0.005% P20 was injected at several concentrations for 60 seconds and allowed to dissociate for 60 seconds.
After the end of each injection, antibodies were allowed to dissociate for 20min (1200sec) and then the chip was regenerated with 100mM HCl (BIAcore).
Complexes were allowed to dissociate for approximately three minutes, after which a solution of wtDR1/HAY308A complexes (∼2.5 µM) pre-incubated with SEH or SEA at a 1∶1 molar stoichiometry was injected over the surface of flow cell 1 or 2 respectively for another ten minutes.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com