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Gel electrophoresis, dynamic light scattering (DLS) and zeta potential analyses indicated that polyplexes formed at an N/P ratio of 10/1 were dissociated following 5 h incubation at pH 5.0, highlighting the importance of hydrophobic TMB-THME moieties in DNA condensation and supporting that acetal hydrolysis in endosomes would facilitate DNA release.
"People who were more dissociated following the film didn't show the beneficial effect of the nitrous oxide.
However, while the food-based detection results in Figure 4A were obtained using spiked BoNT/A holotoxin, the toxin is often found in its natural complex form in environmental and food samples (and subsequently dissociated following intestinal absorption).
Planarians that were dissociated following Smed-set1 RNAi showed a decrease in the percentage of cells in the X1 population (the cycling stem cells) from a mean of 14.4% in the gfp RNAi) controls to 5.6% in Smed-set1 RNAi) Smed-set1 RNAi5B animals
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"Some people have a tendency to dissociate following traumatic events," he said.
Furthermore, the beta-gamma subunits of heterotrimeric G-proteins, which dissociate following receptor activation, have their own set of biological activities (e.g. modulation of plasma membrane ion channels).
In fact, pentameric HoxEFUYH hydrogenases tend to dissociate following cell breakage into the diaphorase (Hox-EFU) and hydrogenase (HoxYH) subunits.
The rationale behind this was that such mutants would stay associated for longer rather than merely transiently binding and dissociating following SUMO cleavage.
The assumption was perpetuated due to the suggestion that ANP NPRA complexes rapidly dissociate following ANP binding to NPRA at 37°C.
Furthermore, the stimulation dependence of the RACK1/mAChR interaction is similar to that of the NMDA receptor, where RACK1 acts as an inhibitory scaffolding protein, preventing NMDA receptor phosphorylation until the proteins dissociate following stimulation [28].
After 8-day culture, neurosphere were formed and the cells were enzymatically dissociated for following experiments.
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