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Once dissociated, cells were counted using a hemocytometer and plated at ~ 50,000 cells per well in an 8-well glass bottom chamber slide.
Pool dissociated cells.
In Figure 1, the arrows indicate dissociated cells.
The supernatant spun at 1,200 g for 7 min to collect dissociated cells.
Dissociated cells formed neurospheres after 3 days in culture.
Dissociated cells were centrifuged 5 min at 800 rpm and resuspended in the dissociation medium.
Steinberg, M. S. Reconstruction of tissues by dissociated cells.
Dissociated cells were collected by centrifugation (200 × g for 5 min) and resuspended in chilled culture medium.
The dissociated cells then settle, migrate, and form active aggregates in which the archaeocytes play an important role.
For DNA content analysis, dissociated cells were incubated with the vital DNA marker Hoechst 33342 (Sigma)72.
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We first compared different culture media for ability to maintain the pluripotency of EDTA-dissociated cells prior to differentiation.
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