Briefly, dissociated cells were seeded at 5 × 10/well in a 24-well plate coated with poly- d-lysine.
Briefly, dissociated cells were re-suspended in transfection medium, mixed with the respective siRNAs (200 nM), and electroporated using the fixed program (O-03) for optimal neuronal transfection.
Briefly, dissociated rat neonatal cortices were cultured at 37℃ in 5.0% CO2 in Dulbecco's modified Eagle's medium (DMEM) with 10% fetal calf serum (FCS) and penicillin/streptomycin in Poly-D-lysine (PDL -coated flasks.
Briefly, dissociated cells were washed in rat Ringer's solution and loaded in the dark with Fura-2AM supplemented with pluronic acid in rat Ringer's solution for 45 min at room temperature (RT).
Briefly, dissociated cells were plated onto poly-𝒟-lysine (PDL -coated T75 flasks or 96 well culture PDL -coatedwere fed every oT75r day with D10S (DMEM with 10% flasksor 7–10 days.
Briefly, the dissociated NS cells were cultured and plated as adherent monolayer (7.5 × 10 cells/cm) in DMEM-F12 supplemented only with 10% FCS (FCS medium) on laminin (1.5 μg/cm, Sigma-Aldrich, Saint-Quentin Fallavier, France) for at least 15 days to ensure an optimum differentiation.
Briefly, 1 mL of dissociated immature cortical cells (10 cells) treated with KA at five different concentrations for four different induction times as mentioned above was incubated with fluorescent dye.
Briefly, the brains were freed from meninges, and dissociated mechanically and enzymatically with trypsin and DNAse (Sigma-Aldrich, München, Germany).
Briefly, 1.35±0.73% (n = 3) of the dissociated epidermal cells appeared blue, indicating that an average of 57% of MCs were transfected by this approach.
Briefly, HEK293 cells cultured on 24-well plates or dissociated cortical neurons cultured on 12-well plates, were subjected to 2 h OGD or 2 h OGD plus 24 h reoxygenation.
