It is found that CO2 dissociates via a direct pathway to produce CO and O dominantly, and atomic O is revealed to be the primary oxidant of CHx intermediates.
In the pulsed DBD plasma jet, NO radicals are made through O2 and N2 dissociating via the electron collision process [21].
The second substrate, mal-AcpM, then enters the lateral channel, and is decarboxylated by the catalytic residues His244 and Asn274, and condensed with the thioester formed at Cys112 to generate the β-ketoacyl-AcpM, which also dissociates via the lateral channel.
After 5 h of incubation, the cells were dissociated via proteolytic digestion with papain.
The Yariv AGP complex was then dissociated via reductive cleavage of the diazo linkage with sodium dithionite, followed by dialysis and freeze-drying as described previously (Pal et al. 2003).
To quantify the percentage of apoptotic cells in H-1PV-infected cultures (MOI = 20 PFU/cell), adherent cells were dissociated via trypsinization and collected 3 days p.i. by centrifugation at 800 × g.
For all other antigens, cells were dissociated via Accutase or Versene for 15 30 min, fixed for 15 min with 2% paraformaldehyde or ice-cold methanol, and washed twice with PBS containing 1% BSA.
Briefly, tissue was collected in cold DMEM containing 5 mM glucose and 20 mM Hepes, minced finely with a sterile scalpel, and cells were dissociated via agitated incubation at 37 °C in digestion medium containing 0.25% (w/v) trypsin, 0.1% (w/v) type IV collagenase, and 0.1% (w/v) bovine serum albumin (BSA).
The cells were then enzymatically dissociated via the addition of collagenase type XI 0.2percentt (Sigma, St . Louis MO) for 1 hour at 37°C, 240 units of Grade II dispase (Gibco-BRL, Grand Island, NY) for 45 minutes, and trypsin 0.1percentt (Gibco-BRL) for 30 minutes.
