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The efficiency of polyplex-mediated transfection depends on the ability of the polymer to condense DNA, while allowing it to dissociate once inside the cell.
In contrast, an inhibitor of filopodia initiation would be expected to be present at the ´birthplace´ of filopodia, and to dissociate once elongation/outgrowth begins.
The reduced intensity of the pause prior to the 5′-most AGG sequences could be accounted for by a propensity of the dimer to dissociate once the first of the four strands has been removed from the quadruplex by reverse transcription.
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Promotion should be dissociated once and for all from the ruler's (or his ministers') personal judgments.
Once the cultures stabilized, the spheres were routinely dissociated once a week and plated at 106 cells/75 cml/10 ml.
However, these studies do not provide information on the angular displacement of γ until Pi dissociates once it binds to F1-ATPase as ATP.
RNA DNA heteroduplexes are thermodynamically more stable than the respective DNA strands within the double helix (Roberts and Crothers 1992), especially when the NTS strand is G-rich (Ginno et al. 2013); thus, one might wonder how the cell is able to dissociate them once they form.
Following intra-CSF injection of CFSE-labeled DCs in normal rats (n = 7) or EAE rats (n = 8), CLNs and axillary lymph nodes were mechanically dissociated, washed once in PBS 1× then filtered through a 70 µm cell strainer (Pharmingen, San Diego, USA).
Analysis of the junctional microdomain areas and circumference as a function of time during microdomain dissection revealed that the adhesion function of the proteins was cooperative; entire membrane patches dissociated at once.
If you were popular, your best friends will dissociate from you once you are diagnosed to be positive.
Once dissociated, cells were counted using a hemocytometer and plated at ~ 50,000 cells per well in an 8-well glass bottom chamber slide.
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