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Of these, 430 proteins (10%) are found at the intersection of clusters annotated to dissimilar functions and are considered EMF candidates (Supplementary Data 3).
However, fundamental differences remain as these cells are part of larger organs with dissimilar functions and are situated in very different microenvironments.
The relatively close relationship between the terminal domains of the egg-case silk protein TuSp1 and the orb-web capture spiral silk protein Flag is especially surprising given their dissimilar functions and repetitive sequence properties.
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Finally, to distinguish extreme from 'classical' multifunctional proteins, proteins found at the intersection of functional modules annotated to dissimilar functions were identified.
Most of the candidates (>90%) were found annotated to dissimilar functions involving on the one hand nucleic acid-linked metabolic processes and on the other (i) signalling activity, (ii) localization or (iii) transport (see Table 1 and Supplementary Data 4).
Tandem repeats confer important structural features and special functions to these proteins but they usually occur in association with other domains with specific biological roles, and consequently proteins in the same family may perform dissimilar functions.
In this study, a detailed dimensional analysis was conducted on a selected panel of natural fibres with contrasted morphometric characteristics belonging to various phylogenetic plant species with dissimilar functions in planta.
Furthermore, it has been reported that the developmental and tissue expression patterns of the mH2A subtypes differ, supporting the idea that different subtypes have dissimilar functions.
To understand how kinesins with structurally similar motor domains can have such dissimilar functions, we elucidated the ATP turnover cycle of the kinesin-13, MCAK.
Therefore, the lineage-specific patterns may be explained by the fact that different lineages represent various loci with dissimilar functions [see [ 51]].
A methodological consequence is that, as gene orthology prediction is mostly based on sequence similarity, orthologous genes can be erroneously inferred by grouping the wrong gene isoforms, which might have dissimilar functions.
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