TW performed the study design and managed the study execution, including cadaver dissection, study testing, data analysis, and manuscript writing.
All samples were kept moist during the dissection and testing process.
Avenzoar, an Arabic physician in 12th-century Moorish Spain who also practiced dissection, introduced animal testing as an experimental method of testing surgical procedures before applying them to human patients.
The performance of this "meso" dissection system was tested using post dissection visual examination for resolution and accuracy, fluorescence based DNA quantitation for recovery efficiency, and dissection of closely situated mouse-human tissue sections followed by PCR amplification for purity determination.
NA, not assessed (in controls being head and neck cancer patients without parotid involvement, no functional tests were performed as all these patients were already scheduled for a neck dissection and functional tests were considered too much of a burden to the patients at that time).
However, this normalization also requires precision dissections for each test subject along the rostral-caudal axis, as the inadvertent additions of non-DA cells in the dissection would increase protein content not related to DA neuropil.
TK participated in study design and execution, including cadaver dissection and study testing.
During dissection, preparation, and testing, the specimens were moistened with physiological saline solution to prevent dehydration.
Specimens were kept moist with normal saline solution during all phases of dissection, preparation, and testing.
Specific interactions of interest were further examined by direct mating, tetrad dissection and spot-testing.
