The 3D model of each case was then used to determine the sections and subregions to be dissected, in relation with adjacent cresyl violet-stained sections, which enabled to generate precise templates of dissection of the sections of interest, focusing on focusing on the cortical plate of the PFO and PT domains (cf Figure 1).
In some cases, surgically excised melanomas contain a significant normal tissue component, potentially limiting the biomarker utility of detection of NT5E CpG methylation, but this could be readily overcome by micro-dissection of tissue sections before methylation analysis.
Therefore, direct sequencing requires the condensation of tumor cells by manual dissection of the tissue sections in which tumor cells are densely contained (manual microdissection).
To check the precision of dissection, the macrodissected sections were stained with LFB.
If there was no previous caesarean section, the dissection of the bladder from the cervix is obviated by strong pressure on the uterine instrument to push the uterus into the cranial direction (Fig. 3).
The histology of sections immediately adjacent to the area of the dissection was reviewed and classified as histologically normal or cancerous by a trained pathologist.
The performance of this "meso" dissection system was tested using post dissection visual examination for resolution and accuracy, fluorescence based DNA quantitation for recovery efficiency, and dissection of closely situated mouse-human tissue sections followed by PCR amplification for purity determination.
The previous section showed the dissection of the routes of regulation of the ASA supply block rate by ASA for the wild type pathway.
After dissection of the cerebellum a part of the entorhinal cortex was cut off and the hemispheres were glued with the cross section on the slicing platform of the sectioning system (Vibratome 3000, St . Louis MO, USA).
It uses a stained section to guide the dissection of the unstained adjacent specimen [ 23].
The Section view is a dissection of the structure, as if it were sliced through vertically than opened up so you can see what is inside.
