Human studies have largely employed homogenized brain tissue reflecting multiple cell types, however, complicating the dissection of cell-specific processes that are dysregulated.
For the dissection of cell-autonomous vs non-cell autonomous effects of the loss of kif5aa, we generated mutant/wild-type chimeric embryos by blastomere transplantations at the 1000-cell staGosseossetet al., 2008).
A common problem is suboptimal microscopic visualization because of the absence of mounting medium and a coverslip (Esposito 2007), making precise dissection of cells with a lack of architectural features, such as lymphoid tissues, almost impossible (Fend and Raffeld 2000).
The derivation of germ cells from embryonic stem cells in vitro provides an invaluable assay both for the genetic dissection of germ cell development and for epigenetic reprogramming, and may one day facilitate nuclear transfer technology and infertility treatments.
Genetic dissection of programmed cell death (PCD) was first performed in C. elegans, in which a defined number of cells (131 out of 1090 cells) were revealed to undergo developmentally controlled death (Ellis and Horvitz, 1986).
The specific molecular and biochemical dissection of stem cell self-renewal and differentiation mechanisms have been hampered due to the heterogeneous nature of the neurosphere assay.
In addition to their well-known use in nonlinear imaging [ 1, 2] and medical implant modification [ 3, 4], manipulation and dissection of individual cells in tissue or structures inside living cells and other biological materials can be accomplished using femtosecond pulses with nanoscale precision [ 5, 6].
In this study we have investigated the role of CCR7 in the recruitment of CD4+ memory T cells into the inflamed joints of patients with JIA, and attempted the functional and anatomical dissection of these cells according to their expression of CCR7, CXCR3, CCR5 and IFN-γ.
