All pathology and laboratory procedures (slide review, sectioning of tumor blocks, macro-dissection, RT-PCR assays, and Recurrence Score calculations) were conducted blinded to the case-control status of patient specimens.
Using both adherent monolayer and non-adherent colony assays allowed dissection of important aspects of the interaction between Ewing tumour cells and their microenvironment.
JG co-drafted the manuscript, performed primary cell culture experiments, ROS assays, animal dissections, tumor measurements and performed statistical analyses, and analyzed and interpreted the results.
Here, we describe a microfluidic platform that utilizes well-controlled three-dimensional (3D) diffusion to generate molecular gradients of varied steepness in a large array of hydrogel cylinders, allowing high-throughput 3D chemotactic assays for mechanistic dissection of steepness-dependent neuronal chemotaxis.
and 90 minutes later were sacrificed by decapitation and the hypothalamus was dissected and frozen for subsequent cytokine analysis (see procedures below for dissection and assay details).
The availability of a large number of SNP markers and cost-effective genotyping assays facilitates the genetic dissection of complex traits via genome-wide association studies (GWAS).
We investigated the allelic exclusion/silencing phenotype in in vivo cross-fertilization assays by tightly timing the mosquito midgut dissections and quantifying the number of single- and double-positive parasites in each midgut.
J.I. performed dissections, molecular and proteomic work, prepared recombinant stefin, conducted inhibition assays, and wrote the manuscript.
To determine the viral titer of lung and nose samples, tissue samples were snap-frozen upon dissection and subsequently homogenized in serum-free DMEM medium for plaque reduction assays as described above.
DDC carried out the drug administration, behavioral work, dissections and histology, tissue homogenization and ELISAs, TBARS and Total Antioxidant assays, statistical analyses, and drafted the manuscript.
