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The livers were dissected to two parts.
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Diploid transformants were sporulated and the tetrads dissected to yield four haploids, two with the intein insertion and two without.
A small incision was made and the subcutis was carefully dissected to create two (for mice) or three (for rats) subcutaneous tunnels.
Strain YJS329 was previously cultured in sporulation medium for 5 days, and an ascus with four ascospores was dissected to obtain four haploid strains (named YJSH1-4).
18 months old mice brains were dissected to use one hemisphere for RNA, the other hemisphere for protein extraction.
From each patient, tumor and adjacent normal tissue biopsies were dissected to provide four samples per patient (normal epithelial cells, stroma from normal biopsies, CRC cells and cancer derived stroma).
Brains were blocked (i.e., dissected) to include hippocampal areas, one hemisphere for sagittal orientation, and one hemisphere for coronal.
Papillary muscles from the LV were dissected to yield at least two thin strips (~140 μm diameter, ~800 μm length) with longitudinally oriented parallel fibers, skinned for 2 hr at 22°C, and stored at −20°C.
The eyes were removed, post-fixed for 30 minutes and then retina flat mounts dissected to be post-fixed for one hour on filter paper (to force a flat shape during fixation).
The sections were thawed and the stratum oriens, dentate gyrus and rest of hippocampus were dissected; four to seven sections were dissected from each brain.
Female flies were dissected three to five days after eclosure.
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