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For experiments using dissected brain tissue, 20 brains were dissected per sample and cRNA probes were made by converting the RNA to double stranded cDNA that is flanked by a T7 promoter.
Exactly 30 mosquitoes were dissected per child.
Five larvae were dissected per well.
The average number of lymph nodes dissected per patient was 11.2 (range, 1 60 nodes).
Briefly, five brains were dissected per genotype and placed in 1× NuPage (Invitrogen, Carlsbad, CA) sample buffer with 2-mercaptoethanol.
To obtain polarity and higher resolution information on selected lines, split-GAL4 lines were crossed to pJFRC51-3xUAS-Syt::smGFP-HA in su(Hw attP1; pJFRC225-5xUAS-IVS-myr::smGFP-FLAG in VK00005 and four females brains plus two ventral nerve cords (VNCs) were dissected per line and immunolabeled in 2.0 ml tubes as described below.
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Body wall muscles were dissected as per the previous protocols [44].
Hypoxic Incubation: 10 15 females were dissected as per the standard conditions, then the tube containing Robb's was closed and oocytes were incubated for 30 minutes prior to fixation.
The median number of dissected nodes per patient was 11.
dissected nodes per case (median 21, range 1 108).
We used 40 dissected colonies per pre-coated plate.
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