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Mammary tumor tissue samples, as well as uterus, intestine, and normal mammary glands, were immediately dissected into smaller sections, some of which were fixed in formalin for histological analysis.
Whole UCs were manually dissected into smaller sections (5-6 grams UC tissue per isolation) and plated in polystyrene tissue culture flasks with L-DMEM supplemented with 10% fetal bovine serum (Gibco, Carlsbad, CA, USA) and 1% penicillin/streptomycin (Gibco, Carlsbad, CA, USA) for 7 days in a 37°C humidified environment with 5% CO2 [ 24].
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The remaining muscle was immersed in radioactive I-α-bungarotoxin and the muscle fibre bundles dissected into small sections containing the endplates.
Eight-micrometer-thick paraffin sections were cut, mounted on slides, and used for immunohistochemistry. Brains for frozen sections were dissected into smaller blocks typically 3 5 per hemisphere) and stored in buffered fixative at 4 °C.
For immunoblotting, the tumor primary tissues were grossly dissected into smaller pieces and lysated.
Pulmonary lobes were dissected into smaller pieces and digested in 10 mL collagenase-dispase for 50 min with continuous stirring.
Samples from the other hemisphere are placed in small bijous containing 0.1 M Phosphate Buffer (PB) and dissected into smaller segments.
The weighed tissue debridement sample was dissected into small pieces and placed in a 1.5 ml micro-centrifuge tube.
The body walls of leeches were dissected into small pieces and homogenised in liquid nitrogen with a mortar.
For keratocyte isolation, stromal tissue was dissected into small pieces and placed into 25 cm flasks.
The tissues were dissected into small pieces of 80 mg and immediately frozen in liquid nitrogen.
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