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The frozen samples were dissected into slices of 1-2 mm thickness, and more than 90% of cancerous content was a pre-requisite for microarray experiments.
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The gel of the unfertilized eggs was dissected into 11 slices and that of the fertilized eggs into 5 slices for LC-MS/MS analyses.
Freshly removed liver and lung tissues were dissected into thin slices of approximately 2 mm in thickness and immersed under PBS in a micro chamber on a glass slide for examination.
Paraffin blocks of tumors were dissected into 5-µm slices and processed using standard deparaffinization and rehydration techniques.
At the end of the treatment, samples were fixed, paraffin-included, and dissected into 5-μm slices, which were subjected to immuno-histochemical staining with an antibody targeting the Ki67 proliferation-associated marker.
For histopathological examination, hippocampal en bloc resections were dissected into 5-mm-thick slices along the anterior posterior axis.
Brains were rapidly removed and placed in ice-cold isopentane solution, frozen, and coronally dissected into 50 μm thick slices on a cryostat at 12 levels in 1 mm steps, according to the Paxinos and Watson stereotaxic atlas.
Brains were dissected into 1 mm thick coronal slices and immersion fixed in 4% paraformaldehyde in 0.1 M PBS overnight at 4°C.
After decapitation, brains where dissected into halves.
The medial hippocampi were dissected and cut into slices of 500 μm thickness.
At 10 h after BrdUrd injection the tumours were dissected and cut into slices of about 1 mm thickness.
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